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human-diagnostic ELISAs

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The ELISA tests listed below consist of 29 kits for In Vitro diagnosis of autoimmune diseases and 2 kits for the diagnosis of a Helicobacter pylori infection.

These ELISA kits are CE marked and come in the standard format of 96 analyses. They all use identical general reagents, namely sample buffer, wash buffer, substrate and stop solution. Hence, one and the same sample dilution may be used for multiple assays; e.g. for simultaneous determination of Cardiolipin IgG and IgM.

All solutions are ready to use (exception: 10-fold wash buffer concentrate) and color-coded. The conjugate color (red, yellow or green) indicates the Ig-class detected, IgG, IgA or IgM, respectively. The color intensity of the blue calibrators reflects their corresponding concentration. The negative control is green, the positive red.

The solid phase features break apart microwells for economic use of the kit. It is packed in a foil bag with zip closure, useful for partial consumption of the solid phase. The frame of the microwell plate displays the parameter name.

The sample is serum or plasma10 µL are required throughout. It is diluted 1:100 in the orange coloured sample buffer. 100 µL per microwell is the fixed volume of sample, conjugate, substrate and stop solution.

The tests share one common protocol: 3 x 30 minutes incubation at room temperature and without shaking. Wash steps: unvariably 4 x 350 µL per microwell.

The tests have been validated on the Dynex DS2 system and are accessible to other automated devices. The liquids come in polyethylene bottles with enough volume for automation. The detection system is HRP/TMB, read at 450nm.

The compact outer box is labelled on two adjacent sides for easy test kit identification in fridge stacks. Each product comes with printed Directions for Use (also downloadable), including a Summary Flow Chart on the back, and a kit lot-specific Certificate of Analysis. Most of the tests offer the option of qualitative or quantitative evaluation.

The excellent quality of these products is reflected by a very low number of complaints over the years. Since more than a decade, we regularly participate in several international ring trials (UK: Neqas, US: CAP, Germany: Instand and Referenzinstitut für Bioanalytik) with virtually no deviation of our results from the respective intended response.

Prices and bulk discounts on request – E-Mail

All Directions for Use as pdf – Download page

PDF download of Summary Specification Sheet / Product List

Thrombosis with COVID-19

Created on20. May 2020
Independent studies show increased thrombosis and pulmonary embolism in COVID-19 patients; Measurement of Cardiolipin and ß2-GP1 supports therapy assessment Research ...

Meet us at Medica 2020

Created on19. February 2020
Meet us at Medica 2020 , Düsseldorf, Nov. 16. -19. We would be pleased to talk to you about diagnostic ...

Autoimmune disorders
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Diagnostic: Rheumatology
ProductRef.-no.Antigen(s)Measuring rangeCalibrators & Controls
ANA Screen 6 IgG1811FE00RNP, Sm, SS-A/Ro (60kDa), SS-B/La, Scl-70, Jo-1 (mixture)qualitative (ratio)0, pos., neg.
ANA Screen IgG1801PE00dsDNA, RNP, Sm, SS-A/Ro (60+52kDa), SS-B/La, Scl-70, CENP-B, Jo-1 (mixture)qualitative (ratio)0, pos., neg.
ANA Screen 8 IgG1911FE00dsDNA, RNP, Sm, SS-A/Ro (60kDa), SS-B/La, Scl-70, CENP-B, Jo-1 (mixture)qualitativ (ratio)0, pos., neg.
ANA Profile 8 IgG1711FE00dsDNA, RNP, Sm, SS-A/Ro (60kDa), SS-B/La, Scl-70, CENP-B, Jo-1 (separately)qualitativ (ratio)0, pos., neg.
dsDNA IgG0911FE00Circular plasmid dsDNA0 - 250 IU/mL (CDC)6, pos., neg.
RNP lgG1011FE00U1-RNP-specific human proteins A, C and 68kDa, recombinant0 - 100 U/mL6, pos., neg.
Sm lgG1111FE00Sm-D polypeptide subtypes mixture (bovine thymus)0 - 40 U/mL6, pos., neg.
SS-A/Ro IgG1211FE00SS-A/Ro (60kDa, bovine thymus)0 - 60 U/mL6, pos., neg.
SS-B/La IgG1311FE00human SS-B/La protein, recombinant0 - 60 U/mL6, pos., neg.
Scl-70 IgG1411FE00DNA topoisomerase I, recombinant0 - 60 U/mL6, pos., neg.
CENP-B IgG1511FE00Centromere Protein B, recombinant0 - 60 U/mL6, pos., neg.
Jo-1 IgG1611FE00Human histidyl-tRNA synthetase, recombinant0 - 100 U/mL6, pos., neg.
Diagnostic: Thrombosis
ProductRef.-no.Antigen(s)Measuring rangeCalibrators & Controls
ß2-Glycoprotein 1 IgG0711FE00ß2-Glycoprotein 1 (human)0 - 100 U/mL6, pos., neg.
ß2-Glycoprotein 1 IgM0811FE00ß2-Glycoprotein 1 (human)0 - 100 U/mL6, pos., neg.
ß2-Glycoprotein 1 IgA0611FE00ß2-Glycoprotein 1 (human)0 - 100 U/mL6, pos., neg.
Cardiolipin IgG0411FE00Cardiolipin (bovine heart), ß2-Glycoprotein 1 (human)0 - 100 GPL-U/mL6, pos., neg.
Cardiolipin IgM0511FE00Cardiolipin (bovine heart), ß2-Glycoprotein 1 (human)0 - 100 MPL-U/mL6, pos., neg.
Cardiolipin IgA0311FE00Cardiolipin (bovine heart), ß2-Glycoprotein 1 (human)0 - 100 APL-U/mL6, pos., neg.
Diagnostic: Vasculitis
ProductRef.-no.Antigen(s)Measuring rangeCalibrators & Controls
MPO IgG (p-ANCA)2211FE00Myeloperoxidase (human)0 - 100 U/mL6, pos., neg.
PR3 IgG (c-ANCA)2311FE00Proteinase 3 (human)0 - 100 U/mL6, pos., neg.
PR3 IgG (c-ANCA), capture version2105LE00Proteinase 3 (human)0 - 100 U/mL6, pos., neg.
GBM IgG2411FE00NC-1 domain of alpha3-chain of human collagen type IV, recombinant0 - 100 GPL-U/mL6, pos., neg.
Diagnostic: Gastroenterology
ProductRef.-no.Antigen(s)Measuring rangeCalibrators & Controls
Modified Gliadin Peptide IgA0209HE00Modified (deamidated) gliadin peptide (MGP)0 - 100 U/mL6, pos., neg.
Modified Gliadin Peptide IgG0109HE00Modified (deamidated) gliadin peptide (MGP)0 - 100 U/mL6, pos., neg.
Tissue Transglutaminase IgA2711FE00Human tissue transglutaminase, recombinant0 - 100 U/mL6, pos., neg.
Tissue Transglutaminase IgG2811FE00Human tissue transglutaminase, recombinant0 - 100 GPL-U/mL6, pos., neg.
Epidermal Transglutaminase IgA2912FE00Human tissue transglutaminase, recombinant0 - 70 U/mL6, pos., neg.
Diagnostic: Thyroid
ProductRef.-no.Antigen(s)Measuring rangeCalibrators & Controls
Thyroid Peroxidase IgG2111FE00Human thyroid peroxidase, recombinant0 - 3000 IU/mL6, pos., neg.
Thyroglobulin IgG2011FE00Thyroglobulin (human)0 - 3000 IU/mL6, pos., neg.

Non-autoimmune disorders
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Diagnostic: Infectious Disease
ProductRef.-no.Antigen(s)Measuring rangeCalibrators & Controls
Helicobacter pylori IgG0112FE00purified H. pylori extract with urease, surface antigens and Cag-A protein0 - 300 U/mL6, pos., neg.
Helicobacter pylori IgA0212FE00purified H. pylori extract with urease, surface antigens and Cag-A protein0 - 300 U/mL6, pos., neg.