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human-diagnostic ELISAs

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The ELISA tests listed below consist of 29 kits for In Vitro diagnosis of autoimmune diseases and 2 kits for the diagnosis of a Helicobacter pylori infection.

These ELISA kits are CE marked and come in the standard format of 96 analyses. They all use identical general reagents, namely sample buffer, wash buffer, substrate and stop solution. Hence, one and the same sample dilution may be used for multiple assays; e.g. for simultaneous determination of Cardiolipin IgG and IgM.

All solutions are ready to use (exception: 10-fold wash buffer concentrate) and color-coded. The conjugate color (red, yellow or green) indicates the Ig-class detected, IgG, IgA or IgM, respectively. The color intensity of the blue calibrators reflects their corresponding concentration. The negative control is green, the positive red.

The solid phase features break apart microwells for economic use of the kit. It is packed in a foil bag with zip closure, useful for partial consumption of the solid phase. The frame of the microwell plate displays the parameter name.

The sample is serum or plasma10 µL are required throughout. It is diluted 1:100 in the orange coloured sample buffer. 100 µL per microwell is the fixed volume of sample, conjugate, substrate and stop solution.

The tests share one common protocol: 3 x 30 minutes incubation at room temperature and without shaking. Wash steps: unvariably 4 x 350 µL per microwell.

The tests have been validated on the Dynex DS2 system and are accessible to other automated devices. The liquids come in polyethylene bottles with enough volume for automation. The detection system is HRP/TMB, read at 450nm.

The compact outer box is labelled on two adjacent sides for easy test kit identification in fridge stacks. Each product comes with printed Directions for Use (also downloadable), including a Summary Flow Chart on the back, and a kit lot-specific Certificate of Analysis. Most of the tests offer the option of qualitative or quantitative evaluation.

The excellent quality of these products is reflected by a very low number of complaints over the years. Since more than a decade, we regularly participate in several international ring trials (UK: Neqas, US: CAP, Germany: Instand and Referenzinstitut für Bioanalytik) with virtually no deviation of our results from the respective intended response.

Prices and bulk discounts on request – E-Mail

All Instructions for Use as pdf – Download page

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Autoimmune disorders
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Diagnostic: Rheumatology
ProductRef.-no.Antigen(s)Measuring rangeCalibrators & Controls
ANA Screen 6 IgG ELISA1811FE00RNP, Sm, SS-A/Ro (60kDa), SS-B/La, Scl-70, Jo-1 (mixture)qualitative (ratio)0, pos., neg.
ANA Screen IgG ELISA1801PE00dsDNA, RNP, Sm, SS-A/Ro (60+52kDa), SS-B/La, Scl-70, CENP-B, Jo-1 (mixture)qualitative (ratio)0, pos., neg.
ANA Screen 8 IgG ELISA1911FE00dsDNA, RNP, Sm, SS-A/Ro (60kDa), SS-B/La, Scl-70, CENP-B, Jo-1 (mixture)qualitativ (ratio)0, pos., neg.
ANA Profile 8 IgG ELISA1711FE00dsDNA, RNP, Sm, SS-A/Ro (60kDa), SS-B/La, Scl-70, CENP-B, Jo-1 (separately)qualitativ (ratio)0, pos., neg.
dsDNA IgG ELISA0911FE00Circular plasmid dsDNA0 - 250 IU/mL (CDC)6, pos., neg.
U1-RNP lgG ELISA1011FE00U1-RNP-specific human proteins A, C and 68kDa, recombinant0 - 100 U/mL6, pos., neg.
Sm lgG ELISA1111FE00Sm-D polypeptide subtypes mixture (bovine thymus)0 - 40 U/mL6, pos., neg.
SS-A/Ro IgG ELISA1211FE00SS-A/Ro (60kDa, bovine thymus)0 - 60 U/mL6, pos., neg.
SS-B/La IgG ELISA1311FE00human SS-B/La protein, recombinant0 - 60 U/mL6, pos., neg.
Scl-70 IgG ELISA1411FE00DNA topoisomerase I, recombinant0 - 60 U/mL6, pos., neg.
CENP-B IgG ELISA1511FE00Centromere Protein B, recombinant0 - 60 U/mL6, pos., neg.
Jo-1 IgG ELISA1611FE00Human histidyl-tRNA synthetase, recombinant0 - 100 U/mL6, pos., neg.
Diagnostic: Thrombosis
ProductRef.-no.Antigen(s)Measuring rangeCalibrators & Controls
Beta-2 GP1 IgG ELISA0711FE00ß2-Glycoprotein 1 (human)0 - 100 U/mL6, pos., neg.
Beta-2 GP1 IgM ELISA0811FE00ß2-Glycoprotein 1 (human)0 - 100 U/mL6, pos., neg.
Cardiolipin IgG ELISA0411FE00Cardiolipin (bovine heart), ß2-Glycoprotein 1 (human)0 - 100 GPL-U/mL6, pos., neg.
Cardiolipin IgM ELISA0511FE00Cardiolipin (bovine heart), ß2-Glycoprotein 1 (human)0 - 100 MPL-U/mL6, pos., neg.
Diagnostic: Vasculitis
ProductRef.-no.Antigen(s)Measuring rangeCalibrators & Controls
MPO IgG ELISA2211FE00Myeloperoxidase (human)0 - 100 U/mL6, pos., neg.
PR3 capture lgG ELISA2105LE00Proteinase 3 (human)0 - 100 U/mL6, pos., neg.
Diagnostic: Gastroenterology
ProductRef.-no.Antigen(s)Measuring rangeCalibrators & Controls
MGP IgA ELISA0209HE00Modified (deamidated) gliadin peptide (MGP)0 - 100 U/mL6, pos., neg.
MGP IgG ELISA0109HE00Modified (deamidated) gliadin peptide (MGP)0 - 100 U/mL6, pos., neg.
tTG IgA ELISA2711FE00Human tissue transglutaminase, recombinant0 - 100 U/mL6, pos., neg.
tTG IgG ELISA2811FE00Human tissue transglutaminase, recombinant0 - 100 U/mL6, pos., neg.
Diagnostic: Thyroid
ProductRef.-no.Antigen(s)Measuring rangeCalibrators & Controls
TPO lgG ELISA2111FE00Human thyroid peroxidase, recombinant0 - 3000 IU/mL6, pos., neg.
TG IgG ELISA2011FE00Thyroglobulin (human)0 - 3000 IU/mL6, pos., neg.

Non-autoimmune disorders
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Diagnostic: Infectious Disease
ProductRef.-no.Antigen(s)Measuring rangeCalibrators & Controls
H. pylori IgG ELISA0112FE00purified H. pylori extract with urease, surface antigens and Cag-A protein0 - 300 U/mL6, pos., neg.
H. pylori IgA ELISA0212FE00purified H. pylori extract with urease, surface antigens and Cag-A protein0 - 300 U/mL6, pos., neg.