deutsch | english
Products | Download | Order | Contact
Human-diagnostic ELISAs | Streptavidin | pedagogic ELISA | ELISA Development/Production
 > products > human-diagnostic ELISAs

     

Human-diagnostic ELISAs

The ELISA tests listed below consist of 29 kits for In Vitro diagnosis of autoimmune diseases and 2 kits for the diagnosis of a Helicobacter pylori infection.

These ELISA kits are CE marked and come in the standard format of 96 analyses. They all use identical general reagents, namely sample buffer, wash buffer, substrate and stop solution. Hence, one and the same sample dilution may be used for multiple assays; e.g. for simultaneous determination of Cardiolipin IgG and IgM.

All solutions are ready to use (exception: 10-fold wash buffer concentrate) and color-coded. The conjugate color (red, yellow or green) indicates the Ig-class detected, IgG, IgA or IgM, respectively. The color intensity of the blue calibrators reflects their corresponding concentration. The negative control is green, the positive red.

The solid phase features break apart microwells for economic use of the kit. It is packed in a foil bag with zip closure, useful for partial consumption of the solid phase. The frame of the microwell plate displays the parameter name.

The sample is serum or plasma; 10 µL are required throughout. It is diluted 1:100 in the orange coloured sample buffer. 100 µL per microwell is the fixed volume of sample, conjugate, substrate and stop solution.

The tests share one common protocol: 3 x 30 minutes incubation at room temperature and without shaking. Wash steps: unvariably 4 x 350 µL per microwell.

The tests have been validated on the Dynex DS2 system and are accessible to other automated devices. The liquids come in polyethylene bottles with enough volume for automation. The detection system is HRP/TMB, read at 450nm.

The compact outer box is labelled on two adjacent sides for easy test kit identification in fridge stacks. Each product comes with printed Directions for Use (also downloadable), including a Summary Flow Chart on the back, and a kit lot-specific Certificate of Analysis. Most of the tests offer the option of qualitative or quantitative evaluation.

The excellent quality of these products is reflected by a very low number of complaints over the years. Since more than a decade, we regularly participate in several international ring trials (UK: Neqas, US: CAP, Germany: Instand and Referenzinstitut für Bioanalytik) with virtually no deviation of our results from the respective intended response.

Prices and bulk discounts on request - E-Mail

All Directions for Use as pdf - Download page

PDF download of Summary Specification Sheet / Product List

 

Autoimmune disorders
Diagnostics Product Ref.- no. Antigen(s) Measuring range Calibrators & Controls
Rheumatology ANA Screen 6 IgG 1811FE00 RNP, Sm, SS-A/Ro (60kDa), SS-B/La, Scl-70, Jo-1 (mixture) qualitative (ratio) 0, pos., neg.
ANA Screen IgG 1801PE00 dsDNA, RNP, Sm, SS-A/Ro (60+52kDa), SS-B/La, Scl-70, CENP-B, Jo-1 (mixture) qualitative (ratio) 0, pos., neg.
ANA Screen 8 IgG 1911FE00 dsDNA, RNP, Sm, SS-A/Ro (60kDa), SS-B/La, Scl-70, CENP-B, Jo-1 (mixture) qualitative (ratio) 0, pos., neg.
ANA Profile 8 IgG 1711FE00 dsDNA, RNP, Sm, SS-A/Ro (60kDa), SS-B/La, Scl-70, CENP-B, Jo-1 (separately) qualitative (ratio) 0, pos., neg.
dsDNA IgG 0911FE00 Circular plasmid dsDNA 0 - 250 IU/mL (CDC) 6, pos., neg.
RNP IgG 1011FE00 U1-RNP-specific human proteins A, C and 68kDa, recombinant 0 - 100 U/mL 6, pos., neg.
Sm IgG 1111FE00 Sm-D polypeptide subtypes mixture (bovine thymus) 0 - 40 U/mL 6, pos., neg.
SS-A/Ro IgG 1211FE00 SS-A/Ro (60kDa, bovine thymus) 0 - 60 U/mL 6, pos., neg.
SS-B/La IgG 1311FE00 human SS-B/La protein, recombinant 0 - 60 U/mL 6, pos., neg.
Scl-70 IgG 1411FE00 DNA topoisomerase I, recombinant 0 - 60 U/mL 6, pos., neg.
CENP-B IgG 1511FE00 Centromere Protein B, recombinant 0 - 60 U/mL 6, pos., neg.
Jo-1 IgG 1611FE00 Human histidyl-tRNA synthetase, recombinant 0 - 100 U/mL 6, pos., neg.
 
Thrombosis ß2-Glycoprotein 1 IgG 0711FE00 ß2-Glycoprotein 1 (human) 0 - 100 U/mL 6, pos., neg.
ß2-Glycoprotein 1 IgM 0811FE00 2-Glycoprotein 1 (human) 0 - 100 U/mL 6, pos., neg.
ß2-Glycoprotein 1 IgA 0611F 2-Glycoprotein 1 (human) 0 - 100 U/mL 6, pos., neg.
Cardiolipin IgG 0411FE00 Cardiolipin (bovine heart), 2-Glycoprotein 1 (human) 0 - 100 GPL-U/mL 6, pos., neg.
Cardiolipin IgM 0511FE00 Cardiolipin (bovine heart), 2-Glycoprotein 1 (human) 0 - 100 MPL-U/mL 6, pos., neg.
Cardiolipin IgA 0311FE00 Cardiolipin (bovine heart), 2-Glycoprotein 1 (human) 0 - 100 APL-U/mL 6, pos., neg.
 
Vasculitis MPO IgG (p-ANCA) 2211FE00 Myeloperoxidase (human) 0 - 100 U/mL 6, pos., neg.
PR3 IgG (c-ANCA) 2311FE00 Proteinase 3 (human) 0 - 100 U/mL 6, pos., neg.
PR3 IgG (c-ANCA), capture version 2105LE00 Proteinase 3 (human) 0 - 100 U/mL 6, pos., neg.
GBM IgG 2411FE00 NC-1 domain of alpha3-chain of human collagen type IV, recombinant 0 - 100 U/mL 6, pos., neg.
 
Gastroenterology Modified Gliadin Peptide IgA 0209HE00 Modified (deamidated) gliadin peptide (MGP) 0 - 100 U/mL 6, pos., neg.
Modified Gliadin Peptide IgG 0109HE00 Modified (deamidated) gliadin peptide (MGP) 0 - 100 U/mL 6, pos., neg.
Tissue Transglutaminase IgA 2711FE00 Human tissue transglutaminase, recombinant 0 - 100 U/mL 6, pos., neg.
Tissue Transglutaminase IgG 2811FE00 Human tissue transglutaminase, recombinant 0 - 100 U/mL 6, pos., neg.
Epidermal Transglutaminase IgA 2912FE00 Human tissue transglutaminase, recombinant 0 - 70 U/mL 6, pos., neg.
 
Thyroid Thyroid Peroxidase IgG 2111FE00 Human thyroid peroxidase, recombinant 0 - 3000 IU/mL 6, pos., neg.
Thyroglobulin IgG 2011FE00 Thyroglobulin (human) 0 - 3000 IU/mL 6, pos., neg.
Non-autoimmune disorders
Infectious Disease Helicobacter pylori IgG 0112FE00 purified H. pylori extract with urease, surface antigens and Cag-A protein 0 - 300 U/mL 6, pos., neg.
Helicobacter pylori IgA 0212FE00 purified H. pylori extract with urease, surface antigens and Cag-A protein 0 - 300 U/mL 6, pos., neg.


 back | top | terms | data privacy statement | imprint | © 2005 - 2018